浮游植物metacaspase的分布、结构及其功能特性     

苏金净  蔡伟聪  李桂玲  李健  曾珺  刘静雯 《海洋与湖沼》2019,50(5):1059-1069

精氨酸/赖氨酸特异性半胱氨酸蛋白酶(Metacaspase)在浮游植物程序性细胞死亡(programmed cell death.PCD)过程中起着关键作用。本文利用生物信息学方法分析metacaspase基因在浮游植物中的分布,并以海洋球石藻(Emilianiahuxleyi)metacaspases(EhMCs)为代表分析其结构和功能,依据Uniprot和String数据库挖掘与metacaspase功能相关并参与浮游植物PCD过程的互作蛋白。结果发现:共计29条、四种类型的metacaspases分布于11种浮游植物中,它们的metacaspase蛋白一级结构存在明显差异,但位于p20的组氨酸和半胱氨酸活性位点均具有高度的保守性;EhMCs三级结构与酵母的metacaspase十分相似,暗示它们具有类似的功能;浮游植物metacaspases与多种蛋白存在直接或间接的互作关系,在细胞PCD过程、响应环境胁迫和能量代谢等方面发挥重要作用。另外.metacaspase的磷酸化修饰可能在浮游植物PCD信号转导过程中起调节作用。  相似文献   

Geostatistical features of streambed vertical hydraulic conductivities in Frenchman Creek Watershed in Western Nebraska     

Olufemi P. Abimbola  Aaron R. Mittelstet  Troy E. Gilmore 《水文研究》2020,34(16):3481-3491

This study evaluated the spatial variability of streambed vertical hydraulic conductivity (K_v) in different stream morphologies in the Frenchman Creek Watershed, Western Nebraska, using different variogram models. Streambed K_v values were determined in situ using permeameter tests at 10 sites in Frenchman, Stinking Water and Spring Creeks during the dry season at baseflow conditions. Measurements were taken both in straight and meandering stream channels during a 5 day period at similar flow conditions. Each test site comprised of at least three transects and each transect comprised of at least three K_v measurements. Linear, Gaussian, exponential and spherical variogram models were used with Kriging gridding method for the 10 sites. As a goodness-of-fit statistic for the variogram models, cross-validation results showed differences in the median absolute deviation and the standard deviation of the cross-validation residuals. Results show that using the geometric means of the 10 sites for gridding performs better than using either all the K_v values from the 93 permeameter tests or 10 K_v values from the middle transects and centre permeameters. Incorporating both the spatial variability and the uncertainty involved in the measurement at a reach segment can yield more accurate grid results that can be useful in calibrating K_v at watershed or sub-watershed scales in distributed hydrological models.  相似文献   

Moving from plot-based to hillslope-scale assessments of savanna vegetation structure with long-range terrestrial laser scanning (LR-TLS)     

《International Journal of Applied Earth Observation and Geoinformation》2020

Reliable quantification of savanna vegetation structure is critical for accurate carbon accounting and biodiversity assessment under changing climate and land-use conditions. Inventories of fine-scale vegetation structural attributes are typically conducted from field-based plots or transects, while large-area monitoring relies on a combination of airborne and satellite remote sensing. Both of these approaches have their strengths and limitations, but terrestrial laser scanning (TLS) has emerged as the benchmark for vegetation structural parameterization – recording and quantifying 3D structural detail that is not possible from manual field-based or airborne/spaceborne methods. However, traditional TLS approaches suffer from similar spatial constraints as field-based inventories. Given their small areal coverage, standard TLS plots may fail to capture the heterogeneity of landscapes in which they are embedded. Here we test the potential of long-range (>2000 m) terrestrial laser scanning (LR-TLS) to provide rapid and robust assessment of savanna vegetation 3D structure at hillslope scales. We used LR-TLS to sample entire savanna hillslopes from topographic vantage points and collected coincident plot-scale (1 ha) TLS scans at increasing distances from the LR-TLS station. We merged multiple TLS scans at the plot scale to provide the reference structure, and evaluated how 3D metrics derived from LR-TLS deviated from this baseline with increasing distance. Our results show that despite diluted point density and increased beam divergence with distance, LR-TLS can reliably characterize tree height (RMSE = 0.25–1.45 m) and canopy cover (RMSE = 5.67–15.91%) at distances of up to 500 m in open savanna woodlands. When aggregated to the same sampling grain as leading spaceborne vegetation products (10–30 m), our findings show potential for LR-TLS to play a key role in constraining satellite-based structural estimates in savannas over larger areas than traditional TLS sampling can provide.  相似文献   

Exploration prospects of oil and gas in the Northwestern part of the Offshore Indus Basin,Pakistan     

《China Geology》2020,3(4):633-642

Oil and gas resources are short in Pakistan and no commercially viable oil and gas sources have been yet discovered in its offshore areas up to now. In this study, the onshore-offshore stratigraphic correlation and seismic data interpretation were conducted to determine the oil and gas resource potential in the Offshore Indus Basin, Pakistan. Based on the comprehensive analysis of the results and previous data, it is considered that the Cretaceous may widely exist and three sets of source rocks may be developed in the Offshore Indus Basin. The presence of Miocene mudstones has been proven by drilling to be high-quality source rocks, while the Cretaceous and Paleocene–Eocene mudstones are potential source rocks. Tectonic-lithologic traps are developed in the northwestern part of the basin affected by the strike-slip faults along Murray Ridge. Furthermore, the Cretaceous and Paleocene–Eocene source rocks are thick and are slightly affected by volcanic activities. Therefore, it can be inferred that the northwestern part of Offshore Indus Basin enjoys good prospects of oil and gas resources.  相似文献   

基于线粒体控制区序列的花斑蛇鲻遗传多态性分析     

李敏  孔啸兰  许友伟  陈作志 《热带海洋学报》2020,39(4):42-49

花斑蛇鲻(Saurida undosquamis)是一种重要的底层经济鱼类, 本研究利用线粒体控制区(D-loop区)序列分析了中国近海分布的花斑蛇鲻的遗传结构和遗传多样性, 一共测定了6个地理群体129尾样本的D-loop区全序列。结果显示, 全长为921bp的序列包含71个多态性位点, 共检测到101个单倍型。花斑蛇鲻总体呈现很高的单倍型多样性(0.9873 ± 0.0048)和较低的核苷酸多样性(0.0132 ± 0.0067)的特征。基于邻接法构建的单倍型系统发育树的拓扑结构很浅, 没有形成分化明显的支系。单倍型在各个地理群体中的分布呈分散交叉状态, 表明地理群体间没有显著的遗传分化。6个群体的分子方差分析显示, 花斑蛇鲻绝大部分的遗传变异(99.87%)来源于群体内的个体之间, 而群体间的变异仅占0.13%。大部分地理群体之间的遗传分化指数(F_ST)均很小, 揭示了群体间的基因交流很频繁, 存在高度的遗传同质性。这些研究结果表明中国近海的花斑蛇鲻遗传多样性丰富, 没有明显的遗传分化, 是一个随机交配群, 可以作为同一个渔业单元来管理。  相似文献   

基于SLAF测序分析3个塔形马蹄螺群体的遗传结构和多样性*     

黄景  欧泽奎  刘文广  何毛贤 《热带海洋学报》2020,39(5):1-18

塔形马蹄螺(Tectus pyramis), 海洋软体动物, 广泛分布于中国深圳大鹏湾、海南岛、西沙和南沙群岛的珊瑚礁生态系统中。文章采用特异性位点扩增片段简化基因组测序技术(SLAF-seq)对来自深圳(SZ)、三亚(SY)和西沙(XS)的塔形马蹄螺群体的遗传多样性和结构进行分析。通过测序共获得115.74Mb读长数据, 平均测序深度为337.87倍, 测序质量值(Q₃₀)平均为94.07%。研究获得118679个多态性SLAF标签, 获得846502个高度一致的群体单核苷酸多态性位点(SNPs), 其中有155个显著差异SNPs。3个群体的平均观察杂合度(H_o)和期望杂合度(H_e)值分别为0.1441~0.1611和0.2537~0.2695。平均多态性信息含量(PIC)为0.2048~0.2176。通过系统发育树分析、主成分分析和聚类分析, 将45个个体大致分为3个类群, 每个群体的所有个体都可以聚类成一个类群。SZ和SY群体的遗传结构相似, 遗传距离最低(0.2510), 遗传分化系数F_st值最低(0.0818)。但XS与其他两个群体间的遗传分化明显, 尤其是XS和SZ群体(F_st=0.1868)。3个群体的遗传分化可能与海流和地理位置有关。  相似文献   

燕山中部大地热流及岩石圈热结构特征——以承德市七家- 茅荆坝地热田为例     

刘峰  王贵玲  张薇  岳晨  陆川  黄起伟  欧小科 《地质学报》2020,94(7):1950-1959

燕山中部地区地热资源丰富,地热地质条件较好,但该地区大地热流测量工作较少,岩石圈热结构研究尚未开展,制约了该地区的地热地质研究与资源勘探开发。本文以该地区的七家-茅荆坝地热田为典型区,结合地温测井、取样测试、数据收集与分析,初步查明燕山中部大地热流特征及浅部-深部岩层热物性特征,填补了大地热流值测量空白区,在此基础上建立了研究区热结构概念模型,估算其深部地温分布。研究得出燕山中部大地热流值变化较大,平均约57 mW/m~2,与周边温泉水温存在较好的相关关系。其中七家-茅荆坝地热田大地热流值较高,为74.9 mW/m~2。通过深部地温分布计算得出七家—茅荆坝地区居里面埋深为21.5～22.8 km,莫霍面温度约815℃,分析结果与前人研究成果较为一致,验证了本文所建立岩石圈热结构模型的准确性。  相似文献   

浙南花岗岩残积土物质结构及工程地质特性研究     

孙强  张泰丽  伍剑波  王赫生 《地质论评》2020,66(S1):163-166

  相似文献   

The structure,depositional style and accumulation characteristics of continental margin with diachronous breakup in the northern South China Sea     

Yunfan Zhang  Jiangyang Zhang  Lijie Wang 《International Geology Review》2020,62(7-8):1006-1018

ABSTRACT

A typical diachronous breakup developed in the South China Sea (SCS); the breakup has been unequivocally identified, and gradually decreases in age from NE to SW. To illustrate the influence of a diachronous breakup on hydrocarbon accumulation, we used seismic profiles from CNOOC, and analysed crustal extension characteristics, sedimentary style and source rock accumulation. The main conclusions are as follows: (1) Because of the diachronous breakup in the SCS, various tectonic units developed in different positions on the northern continental margin with different degrees of extension. The Pearl River Mouth Basin (PRMB) is closer to the central sea basin of the SCS and has been affected by regional tension stress for a longer time. Tectonic units of proximal domain, necking zone, and distal margin types developed, and the crust structure thinned from the land towards the sea. The Qiongdongnan Basin (QB) is closer to the Southwest Sub-basin. There are symmetrical proximal domains on the southern and northern sides of the QB, with a necking zone in the central part. It presents as an abandoned passive continental margin. (2) The PRMB exhibited rifted cells first, followed by canyon type extensional faulting and horizontal axial transportation, and then by a longitudinal shelf-slope sedimentary style. But the QB has only rifted cells and canyon type faulting stages, no longitudinal shelf-slope sedimentary stage. The major source rocks in the northern SCS become younger from east to west, and the sedimentary facies change from lacustrine facies to marine-continental transitional facies. (3) The diachronous breakup process ultimately affecting oil and gas migration and accumulation process. The PRMB is dominated by deep-water shelf break accumulation with deep-water fan reservoir bodies. The QB is dominated by canyon type accumulation with channels.  相似文献   

圆筒型FPSO优化设计与水动力性能分析     

赵治民  李焱  唐友刚  何鑫  童波 《海洋工程》2020,38(1):11-19

针对圆筒型海上储油装置FPSO垂荡运动性能较差、无法安装干式井口的问题,设计了带延伸筒体与矩形阻尼结构的圆筒型FPSO,根据延伸筒体与矩形阻尼结构是否通海分为两种型式。建立水动力计算模型,比较分析不同延伸筒体和阻尼结构型式对FPSO水动力性能的影响。针对南海作业海域,设计了悬链式系泊系统,基于JONSWAP波浪谱对FPSO的运动进行时域预报,并对系泊系统进行校核。分析结果表明:通海型FPSO垂荡固有周期显著提升,可以错开南海百年一遇谱峰周期,通海型FPSO满足钻井、安装干式井口的运动响应要求,系泊系统系缆张力满足规范要求。  相似文献